- CITATIONS: 1
|Application ||WB, FC, E|
|Other Accession||Q568Y3, Q91YT2, Q6PC78, Q5ZIR9, NP_689480.2|
|Reactivity||Human, Mouse, Hamster|
|Predicted||Chicken, Zebrafish, Rat|
|Calculated MW||20459 Da|
|Antigen Region||88-117 aa|
|Other Names||E3 ubiquitin-protein ligase RNF185, 632-, RING finger protein 185, RNF185|
|Target/Specificity||This RN185 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human RN185.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RN185 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed:21931693). Acts in the endoplasmic reticulum (ER)- associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed:27485036). Protects cells from ER stress-induced apoptosis (PubMed:27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed:24019521). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed:24019521).|
|Cellular Location||Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein|
|Tissue Location||Ubiquitously expressed.|
Provided below are standard protocols that you may find useful for product applications.
Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :
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