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RN185 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RN185 Antibody  (Center) AP10818c
    RN185 Antibody (Center) (Cat. #AP10818c) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).
  • WB - RN185 Antibody  (Center) AP10818c
    RN185 Antibody (Center) (Cat. #AP10818c) western blot analysis in mouse kidney tissue and K562 cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).
  • FC - RN185 Antibody  (Center) AP10818c
    RN185 Antibody (Center) (Cat. #AP10818c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96GF1
Other Accession Q568Y3, Q91YT2, Q6PC78, Q5ZIR9, NP_689480.2
Reactivity Human, Mouse, Hamster
Predicted Chicken, Zebrafish, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 20459 Da
Antigen Region 88-117 aa
Additional Information
Gene ID 91445
Other Names E3 ubiquitin-protein ligase RNF185, 632-, RING finger protein 185, RNF185
Target/Specificity This RN185 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human RN185.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRN185 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF185 {ECO:0000303|Ref.1, ECO:0000312|HGNC:HGNC:26783}
Function E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed:21931693). Acts in the endoplasmic reticulum (ER)- associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed:27485036). Protects cells from ER stress-induced apoptosis (PubMed:27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed:24019521). Also acts as a regulator of the innate antiviral response by catalyzing 'Lys-27'-linked polyubiquitination of CGAS at 'Lys-173' and 'Lys-384', thereby promoting CGAS cyclic GMP-AMP synthase activity (PubMed:28273161). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed:24019521).
Cellular Location Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Location Ubiquitously expressed.
Research Areas
Citations ( 0 )


Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :

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$ 365.00
$ 140.00
Cat# AP10818c
Availability: In Stock
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