THUM3 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| IHC-P, WB, E |
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Primary Accession | Q9BV44 |
Other Accession | NP_056268.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 57003 Da |
Antigen Region | 194-223 aa |
Gene ID | 25917 |
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Other Names | THUMP domain-containing protein 3, THUMPD3 |
Target/Specificity | This THUM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-223 amino acids from the Central region of human THUM3. |
Dilution | IHC-P~~1:50~100 WB~~1:1000 E~~Use at an assay dependent concentration. |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | THUM3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | THUMPD3 (HGNC:24493) |
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Function | Catalytic subunit of the THUMPD3-TRM112 methyltransferase complex, that specifically mediates the S-adenosyl-L-methionine- dependent N(2)-methylation of guanosine nucleotide at position 6 (m2G6) in tRNAs (PubMed:34669960, PubMed:37283053). This is one of the major tRNA (guanine-N(2))-methyltransferases (PubMed:37283053). Also catalyzes the S-adenosyl-L-methionine-dependent N(2)-methylation of guanosine nucleotide at position 7 of tRNA(Trp) (PubMed:34669960). |
Cellular Location | Cytoplasm |

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Provided below are standard protocols that you may find useful for product applications.
References
Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)

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