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AASS Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AASS Antibody (C-term) AP11094b
    AASS Antibody (C-term) (Cat. #AP11094b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the AASS antibody detected the AASS protein (arrow).
    detail
  • FC - AASS Antibody (C-term) AP11094b
    AASS Antibody (C-term) (Cat. #AP11094b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q9UDR5
Other Accession NP_005754.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 102132 Da
Antigen Region 805-834 aa
Additional Information
Gene ID 10157
Other Names Alpha-aminoadipic semialdehyde synthase, mitochondrial, LKR/SDH, Lysine ketoglutarate reductase, LKR, LOR, Saccharopine dehydrogenase, SDH, AASS
Target/Specificity This AASS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 805-834 amino acids from the C-terminal region of human AASS.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAASS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AASS (HGNC:17366)
Function Bifunctional enzyme that catalyzes the first two steps in lysine degradation.
Cellular Location Mitochondrion.
Tissue Location Expressed in all 16 tissues examined with highest expression in the liver
Research Areas
Citations (0)
citation

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Background

This gene encodes a bifunctional enzyme that catalyzes the first two steps in the mammalian lysine degradation pathway. The N-terminal and the C-terminal portions of this enzyme contain lysine-ketoglutarate reductase and saccharopine dehydrogenase activity, respectively, resulting in the conversion of lysine to alpha-aminoadipic semialdehyde. Mutations in this gene are associated with familial hyperlysinemia.

References

Sacksteder, K.A., et al. Am. J. Hum. Genet. 66(6):1736-1743(2000)
Papes, F., et al. Biochem. J. 344 PT 2, 555-563 (1999) :

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$ 182.50
$ 70.00
Cat# AP11094b
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