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EIF2B4 Antibody (Center K161)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9UI10 |
| Other Accession | Q3T058, NP_001029288 |
| Reactivity | Human, Mouse |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57557 Da |
| Antigen Region | 146-175 aa |
| Gene ID | 8890 |
|---|---|
| Other Names | Translation initiation factor eIF-2B subunit delta, eIF-2B GDP-GTP exchange factor subunit delta, EIF2B4, EIF2BD |
| Target/Specificity | This EIF2B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-175 amino acids from the Central region of human EIF2B4. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | EIF2B4 Antibody (Center K161) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EIF2B4 |
|---|---|
| Synonyms | EIF2BD |
| Function | Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit (PubMed:25858979, PubMed:27023709, PubMed:31048492). Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl- initiator methionine tRNA available to the ribosome and consequently global translation is repressed (PubMed:25858979, PubMed:31048492). |
| Cellular Location | Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q09924} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
References
Naukkarinen, J., et al. PLoS Genet. 6 (6), E1000976 (2010) :
Wu, Y., et al. J. Hum. Genet. 54(2):74-77(2009)
Horzinski, L., et al. PLoS ONE 4 (12), E8318 (2009) :
Pronk, J., et al. Mult. Scler. 14(8):1123-1126(2008)
Wong, S.S., et al. J. Child Neurol. 23(6):710-714(2008)
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