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KCMF1 antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9P0J7 |
| Other Accession | Q1LZE1, NP_064507.3 |
| Reactivity | Human, Mouse |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 41945 Da |
| Antigen Region | 280-308 aa |
| Gene ID | 56888 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase KCMF1, 632-, FGF-induced in gastric cancer, Potassium channel modulatory factor, PCMF, ZZ-type zinc finger-containing protein 1, KCMF1, FIGC, ZZZ1 |
| Target/Specificity | This KCMF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-308 amino acids from the C-terminal region of human KCMF1. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | KCMF1 antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KCMF1 {ECO:0000303|PubMed:38297121, ECO:0000312|HGNC:HGNC:20589} |
|---|---|
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and then transfers it to targeted substrates, promoting their degradation by the proteasome (PubMed:15581609, PubMed:25582440, PubMed:34893540, PubMed:37891180, PubMed:38297121). Together with UBR4, component of the N-end rule pathway: ubiquitinates proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their degradation (PubMed:34893540, PubMed:37891180). Does not ubiquitinate proteins that are acetylated at the N-terminus (PubMed:37891180). Together with UBR4, part of a protein quality control pathway that catalyzes ubiquitination and degradation of proteins that have been oxidized in response to reactive oxygen species (ROS): recognizes proteins with an Arg-CysO3(H) degron at the N-terminus, and mediates assembly of heterotypic 'Lys-63'-/'Lys-27'-linked branched ubiquitin chains on oxidized proteins, leading to their degradation by autophagy (PubMed:34893540). Catalytic component of the SIFI complex, a multiprotein complex required to inhibit the mitochondrial stress response after a specific stress event has been resolved: ubiquitinates and degrades (1) components of the HRI-mediated signaling of the integrated stress response, such as DELE1 and EIF2AK1/HRI, as well as (2) unimported mitochondrial precursors (PubMed:38297121). Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1 (PubMed:38297121). |
| Cellular Location | Cytoplasm. Late endosome. Lysosome |
| Tissue Location | Spleen, small intestine, ovary, peripheral blood, lung, kidney and pancreas. Expressed at low levels in the thymus, prostate, testis, colon, heart, brain, placenta and liver |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination.
References
Kreppel, M., et al. Oncogene 25(19):2795-2800(2006)
Jang, J.H. FEBS Lett. 578 (1-2), 21-25 (2004) :
Li, Z., et al. Biochem. Biophys. Res. Commun. 306(3):623-628(2003)
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$ 150.00
$ 385.00
Cat# AP11534B
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