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TUBB8B Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - TUBB8B Antibody (C-term) AP11654b
    Confocal immunofluorescent analysis of TUBB8B Antibody (C-term) (Cat#AP11654b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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  • WB - TUBB8B Antibody (C-term) AP11654b
    TUBB8B Antibody (C-term) (Cat. #AP11654b) western blot analysis in K562,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the TUBB8B antibody detected the TUBB8B protein (arrow).
    detail
  • IHC-P - TUBB8B Antibody (C-term) AP11654b
    TUBB8B Antibody (C-term) (Cat. #AP11654b)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TUBB8B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - TUBB8B Antibody (C-term) AP11654b
    TUBB8B Antibody (C-term) (Cat. #AP11654b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession A6NNZ2 
Other Accession A6NNZ2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 253-280 aa
Additional Information
Other Names Tubulin beta 8B
Target/Specificity This TUBB8B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-280 amino acids from the C-terminal region of human TUBB8B.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUBB8B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)
citation

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Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

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$ 150.00
$ 385.00
Cat# AP11654b
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