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GLUD1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P00367 |
| Other Accession | P49448, P10860, P26443, P00368, P00366, NP_005262.1, Q6P3L9, P42174 |
| Reactivity | Human, Mouse |
| Predicted | Zebrafish, Bovine, Chicken, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 61398 Da |
| Antigen Region | 438-465 aa |
| Gene ID | 2746 |
|---|---|
| Other Names | Glutamate dehydrogenase 1, mitochondrial, GDH 1, GLUD1, GLUD |
| Target/Specificity | This GLUD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-465 amino acids from the C-terminal region of human GLUD1. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GLUD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GLUD1 |
|---|---|
| Synonyms | GLUD |
| Function | Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed:11032875, PubMed:11254391, PubMed:16023112, PubMed:16959573). Plays a role in insulin homeostasis (PubMed:11297618, PubMed:9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity). |
| Cellular Location | Mitochondrion. Endoplasmic reticulum. Note=Mostly translocates into the mitochondria, only a small amount of the protein localizes to the endoplasmic reticulum. |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans.
References
Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010)
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Flanagan, S.E., et al. Eur. J. Endocrinol. 162(5):987-992(2010)
Bao, X., et al. J. Neurosci. 29(44):13929-13944(2009)
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