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PIGA Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P37287 |
| Other Accession | NP_065206.3, NP_002632.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54127 Da |
| Antigen Region | 455-484 aa |
| Gene ID | 5277 |
|---|---|
| Other Names | Phosphatidylinositol N-acetylglucosaminyltransferase subunit A, GlcNAc-PI synthesis protein, Phosphatidylinositol-glycan biosynthesis class A protein, PIG-A, PIGA |
| Target/Specificity | This PIGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-484 amino acids from the C-terminal region of human PIGA. |
| Dilution | IHC-P~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PIGA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PIGA (HGNC:8957) |
|---|---|
| Function | Catalytic subunit of the glycosylphosphatidylinositol-N- acetylglucosaminyltransferase (GPI-GnT) complex that catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol and participates in the first step of GPI biosynthesis. |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12.
References
Borowitz, M.J., et al. Cytometry B Clin Cytom 78(4):211-230(2010)
Peruzzi, B., et al. Mutat. Res. 705(1):3-10(2010)
Araten, D.J., et al. Mutat. Res. 686 (1-2), 1-8 (2010) :
Iida, Y., et al. Blood 83(11):3126-3131(1994)
Ware, R.E., et al. Blood 83(9):2418-2422(1994)
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