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RNH1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, E |
|---|---|
| Primary Accession | P13489 |
| Other Accession | NP_976318.1, NP_976317.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 49973 Da |
| Antigen Region | 425-454 aa |
| Gene ID | 6050 |
|---|---|
| Other Names | Ribonuclease inhibitor, Placental ribonuclease inhibitor, Placental RNase inhibitor, Ribonuclease/angiogenin inhibitor 1, RAI, RNH1, PRI, RNH |
| Target/Specificity | This RNH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-454 amino acids from the C-terminal region of human RNH1. |
| Dilution | WB~~1:1000 IF~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RNH1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RNH1 {ECO:0000303|PubMed:36935417, ECO:0000312|HGNC:HGNC:10074} |
|---|---|
| Function | Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and angiogenin (ANG) (PubMed:12578357, PubMed:14515218, PubMed:3219362, PubMed:3243277, PubMed:3470787, PubMed:9050852). May play a role in redox homeostasis (PubMed:17292889). Required to inhibit the cytotoxic tRNA ribonuclease activity of ANG in the cytoplasm in absence of stress (PubMed:23843625, PubMed:32510170). Relocates to the nucleus in response to stress, relieving inhibition of ANG in the cytoplasm, and inhibiting the angiogenic activity of ANG in the nucleus (PubMed:23843625). |
| Cellular Location | Cytoplasm. Nucleus Note=Localizes in the cytoplasm in absence of stress; translocates to the nucleus in response to stress. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.
References
Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010)
Martins-de-Souza, D., et al. J Neural Transm 116(3):275-289(2009)
Turcotte, R.F., et al. Biochem. Biophys. Res. Commun. 377(2):512-514(2008)
Johnson, R.J., et al. Biochemistry 46(45):13131-13140(2007)
Johnson, R.J., et al. J. Mol. Biol. 368(2):434-449(2007)
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