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ALKBH4 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9NXW9 |
| Other Accession | NP_060091.1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 33838 Da |
| Antigen Region | 88-117 aa |
| Gene ID | 54784 |
|---|---|
| Other Names | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4, 11411-, Alkylated DNA repair protein alkB homolog 4, ALKBH4, ABH4 |
| Target/Specificity | This ALKBH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human ALKBH4. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ALKBH4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ALKBH4 {ECO:0000303|PubMed:21166655, ECO:0000312|HGNC:HGNC:21900} |
|---|---|
| Function | Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes (PubMed:23673617). Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (PubMed:23673617). In addition to proteins, also demethylates DNA: specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA, thereby regulating Polycomb silencing (By similarity). |
| Cellular Location | Cytoplasm. Nucleus. Nucleus, nucleolus {ECO:0000250|UniProtKB:Q9D8F1}. Midbody. Note=Associates with the contractile ring and midbody. |
| Tissue Location | Widely expressed, with highest expression in pancreas, ovary and spleen. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).
References
Tsujikawa, K., et al. J. Cell. Mol. Med. 11(5):1105-1116(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
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