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SLC29A3 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9BZD2 |
| Other Accession | NP_060814.4 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 51815 Da |
| Antigen Region | 21-50 aa |
| Gene ID | 55315 |
|---|---|
| Other Names | Equilibrative nucleoside transporter 3, hENT3, Solute carrier family 29 member 3, SLC29A3, ENT3 |
| Target/Specificity | This SLC29A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human SLC29A3. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SLC29A3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SLC29A3 (HGNC:23096) |
|---|---|
| Synonyms | ENT3 |
| Function | Uniporter that mediates the facilitative transport of nucleoside across lysosomal and mitochondrial membranes (PubMed:15701636, PubMed:19164483, PubMed:20595384, PubMed:28729424). Functions as a non-electrogenic Na(+)-independent transporter (PubMed:15701636, PubMed:19164483, PubMed:28729424). Substrate transport is pH-dependent and enhanced under acidic condition, probably reflecting the location of the transporter in acidic intracellular compartments (PubMed:15701636, PubMed:19164483, PubMed:28729424). Proton is not a cotransporting ion but most likely change the ionization state of the transporter which dictates transport- permissible/impermissible conformation for nucleoside translocation (PubMed:28729424). May direct the nucleoside transport from lysosomes to cytosol or cytosol to mitochondria to facilitate the fundamental function of salvage synthesis of nucleic acids (PubMed:28729424). Involved in the transport of nucleosides (adenosine, guanosine, uridine, thymidine, cytidine and inosine) and deoxynucleosides (deoxyadenosine, deoxycytidine) (PubMed:15701636, PubMed:19164483, PubMed:20595384, PubMed:28729424). Also mediates transport of purine nucleobases (adenine, guanine) and pyrimidine nucleobases (uracil) (PubMed:15701636, PubMed:19164483). Also able to transport monoamine neurotransmitters dopamine, serotonin, noradrenaline and tyramine (PubMed:19164483). Capable of transporting ATP (PubMed:19164483). Mediates nucleoside export from lysosomes in macrophages, which regulates macrophage functions and numbers (By similarity). |
| Cellular Location | Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Observed in a punctate intracellular pattern showing partial colocalization with late endosomes/lysosomes (PubMed:15701636). Detected at the cell surface only in certain placental cells (PubMed:19164483) |
| Tissue Location | Widely expressed in both adult and fetal tissues (PubMed:15701636). Highest levels in placenta, uterus, ovary, spleen, lymph node and bone marrow (PubMed:15701636). Expressed in liver (PubMed:19164483). Lowest levels in brain and heart (PubMed:15701636) Expressed in macrophages (PubMed:22174130) |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a nucleoside transporter. The encoded protein plays a role in cellular uptake of nucleosides, nucleobases, and their related analogs. Mutations in this gene have been associated with H syndrome, which is characterized by cutaneous hyperpigmentation and hypertrichosis, hepatosplenomegaly, heart anomalies, and hypogonadism. A related disorder, PHID (pigmented hypertrichosis with insulin-dependent diabetes mellitus), has also been associated with mutations at this locus. Alternatively spliced transcript variants have been described.
References
Gass, N., et al. J Affect Disord 126 (1-2), 134-139 (2010) :
Kang, N., et al. J. Biol. Chem. 285(36):28343-28352(2010)
Li, X., et al. Zhongguo Fei Ai Za Zhi 13(5):458-463(2010)
Priya, T.P., et al. Br. J. Dermatol. 162(5):1132-1134(2010)
Cliffe, S.T., et al. Hum. Mol. Genet. 18(12):2257-2265(2009)
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