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RPS12 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P25398 |
| Other Accession | P47840, P63324, P46405, P63323, P84175, Q76I81, NP_001007.2, G1SFR8 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Chicken, Pig, Rabbit, Rat, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 14515 Da |
| Antigen Region | 15-44 aa |
| Gene ID | 6206 |
|---|---|
| Other Names | 40S ribosomal protein S12, RPS12 |
| Target/Specificity | This RPS12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human RPS12. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RPS12 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RPS12 (HGNC:10385) |
|---|---|
| Function | Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre- rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre- ribosomal RNA by the RNA exosome (PubMed:34516797). Subunit of the 40S ribosomal complex (By similarity). |
| Cellular Location | Cytoplasm. Nucleus, nucleolus |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
References
Yu, Y., et al. Protein Sci. 14(6):1438-1446(2005)
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Sampath, P., et al. Mol. Cell. Biol. 23(5):1509-1519(2003)
Yoshihama, M., et al. Genome Res. 12(3):379-390(2002)
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