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Bcl-2 Antibody (BH3 Domain Specific)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    Confocal immunofluorescent analysis of Bcl-2 Antibody (BH3 Domain Specific) (Cat#AP1303a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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  • WB - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    All lanes : Anti-Bcl-2 Antibody (BH3 Domain Specific) at 1:1000 dilution Lane 1: RPMI 8226 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    Immunohistochemical analysis of AP1303a on paraffin-embedded Human tonsil tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    Immunohistochemical analysis of AP1303a on paraffin-embedded Human placenta tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
  • CITATIONS: 32
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, WB, E
Primary Accession P10415
Other Accession P49950, O02718
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 26266 Da
Antigen Region 75-110 aa
Additional Information
Gene ID 596
Other Names Apoptosis regulator Bcl-2, BCL2
Target/Specificity This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-110 amino acids from human Bcl.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBcl-2 Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL2
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1- inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Cellular Location Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Cytoplasm {ECO:0000250|UniProtKB:P10417}
Tissue Location Expressed in a variety of tissues.
Research Areas
Citations ( 0 )

Background

BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.

References

Zhu, C.J., et al., J. Neurosci. Res. 74(1):60-66 (2003). Scopa, C.D., et al., Dig. Dis. Sci. 48(10):1990-1997 (2003). Grace, V.M., et al., Gynecol. Oncol. 91(1):51-58 (2003). Thoulouze, M.I., et al., Virology 314(2):549-561 (2003). Iwata, A., et al., J. Immunol. 171(6):3136-3141 (2003).

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$ 365.00
$ 140.00
Cat# AP1303a
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