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RPL27 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P61353 |
| Other Accession | P61354, A1XQU5, P61358, Q4R8Z4, Q7ZV82, P61355, P61356, NP_000979.1, G1TXF6 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Chicken, Zebrafish, Monkey, Pig, Rabbit, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 15798 Da |
| Antigen Region | 91-120 aa |
| Gene ID | 6155 |
|---|---|
| Other Names | 60S ribosomal protein L27, RPL27 |
| Target/Specificity | This RPL27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the C-terminal region of human RPL27. |
| Dilution | WB~~1:4000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RPL27 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RPL27 |
|---|---|
| Function | Component of the large ribosomal subunit (PubMed:12962325, PubMed:23636399, PubMed:25901680, PubMed:25957688, PubMed:32669547). Required for proper rRNA processing and maturation of 28S and 5.8S rRNAs (PubMed:25424902). |
| Cellular Location | Cytoplasm, cytosol. Cytoplasm Rough endoplasmic reticulum {ECO:0000250|UniProtKB:A1XQU5} Note=Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity). {ECO:0000250|UniProtKB:A1XQU5, ECO:0000269|PubMed:25957688} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
References
Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Mazumder, B., et al. Cell 115(2):187-198(2003)
Yoshihama, M., et al. Genome Res. 12(3):379-390(2002)
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