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HOOK1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9UJC3 |
| Other Accession | Q8BIL5, NP_056972.1 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 84648 Da |
| Antigen Region | 402-431 aa |
| Gene ID | 51361 |
|---|---|
| Other Names | Protein Hook homolog 1, h-hook1, hHK1, HOOK1 |
| Target/Specificity | This HOOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-431 amino acids from the Central region of human HOOK1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HOOK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HOOK1 (HGNC:19884) |
|---|---|
| Function | Component of the FTS/Hook/FHIP complex (FHF complex) (PubMed:18799622, PubMed:32073997). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) (PubMed:18799622). FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell (PubMed:32073997). Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). |
| Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Note=Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, multivesicular bodies (MVBs), lysosomes, or mitochondria (By similarity). Often found in close association with microtubules (By similarity). Does not associate with the Golgi complex. During spermiogenesis, it localizes to the manchette in spermatids from steps 8-10. It is also present between the microtubule manchette and the nucleus. During manchette elongation, it is preferentially localized to the nuclear ring of the manchette, whereas the strong localization to the manchette decreases. In more mature spermatids, while the manchette migrates posteriorly, it localizes to punctuates spots. At later stages of spermatid differentiation, the punctuate expression pattern is found at both the attachment site and the proximal end of the elongated manchette. In contrast, it is not present in mature spermatozoa (By similarity) {ECO:0000250|UniProtKB:Q8BIL5} |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the hook family of coiled-coil proteins, which bind to microtubules and organelles through their N- and C-terminal domains, respectively. The encoded protein localizes to discrete punctuate subcellular structures, and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq].
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Xu, L., et al. Mol. Biol. Cell 19(12):5059-5071(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Simpson, F., et al. Traffic 6(6):442-458(2005)
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