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TRIM48 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q8IWZ4 |
| Other Accession | NP_077019.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 26409 Da |
| Antigen Region | 42-71 aa |
| Gene ID | 79097 |
|---|---|
| Other Names | Tripartite motif-containing protein 48, RING finger protein 101, TRIM48, RNF101 |
| Target/Specificity | This TRIM48 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-71 amino acids from the N-terminal region of human TRIM48. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TRIM48 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRIM48 (HGNC:19021) |
|---|---|
| Function | E3 ubiquitin-protein ligase which promotes K48-linked polyubiquitination of protein methyltransferase PRMT1, leading to PRMT1 degradation (PubMed:29186683). This suppresses methylation of the PRMT1 substrate MAP3K5/ASK1, promoting its activation and increasing MAP3K5- dependent cell death induced by oxidative stress (PubMed:29186683). TRIM48-mediated ubiquitination of PRMT1 also suppresses methylation of FOXO1 by PRMT1, leading to inhibition of FOXO1 transcriptional activity (PubMed:29186683). |
| Cellular Location | Cytoplasm, cytosol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
TRIM48 belongs to the TRIM/RBCC family. It contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger. There are two named isoforms.
References
Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :
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