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HSPA5 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - HSPA5 Antibody AP1335A
    Confocal immunofluorescent analysis of HSPA5 Antibody(Cat#AP1335a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • WB - HSPA5 Antibody AP1335A
    The anti-HSPA5 Pab (Cat. #AP1335a) is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified Pab.
  • WB - HSPA5 Antibody AP1335A
    Western blot analysis of anti-HSPA5 Pab (Cat. #AP1335a) in HL60 cell line lysates (35ug/lane).HSPA5(arrow) was detected using the purified Pab.
  • IHC-P - HSPA5 Antibody AP1335A
    Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSPA5 antibody (Cat.#AP1335a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IHC-P - HSPA5 Antibody AP1335A
    Formalin-fixed and paraffin-embedded human Testis tissue reacted with HSPA5 antibody (Cat.#AP1335a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - HSPA5 Antibody AP1335A
    HSPA5 Antibody (Cat. #AP1335a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 3
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IF, IHC-P, WB, E
Primary Accession P11021
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 72333 Da
Additional Information
Gene ID 3309
Other Names 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78
Target/Specificity This HSPA5 antibody is generated from rabbits immunized with a recombinant protein encoding full length human HSPA5.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPA5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPA5 (HGNC:5238)
Function Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:2294010, PubMed:23769672, PubMed:23990668, PubMed:28332555). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:1550958, PubMed:19538957). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation (PubMed:26045166).
Cellular Location Endoplasmic reticulum lumen. Melanosome. Cytoplasm {ECO:0000250|UniProtKB:P20029}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Areas
Citations ( 0 )

Background

In cooperation with other chaperones, HSP70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. The HSP70s in mitochondria and the endoplasmic reticulum play an additional role by providing a driving force for protein translocation. They are involved in signal transduction pathways in cooperation with HSP90. They participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

References

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Milner, C.M., et al., Immunogenetics 32(4):242-251 (1990). Sargent, C.A., et al., Proc. Natl. Acad. Sci. U.S.A. 86(6):1968-1972 (1989). Drabent, B., et al., Nucleic Acids Res. 14(22):8933-8948 (1986). Hunt, C., et al., Proc. Natl. Acad. Sci. U.S.A. 82(19):6455-6459 (1985).

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$ 325.00
$ 109.00
Cat# AP1335A
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