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HTRA2 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | O43464 |
| Other Accession | NP_659540.1, NP_037379.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 48841 Da |
| Antigen Region | 73-102 aa |
| Gene ID | 27429 |
|---|---|
| Other Names | Serine protease HTRA2, mitochondrial, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, HTRA2, OMI, PRSS25 |
| Target/Specificity | This HTRA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-102 amino acids from the N-terminal region of human HTRA2. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HTRA2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HTRA2 |
|---|---|
| Synonyms | OMI, PRSS25 |
| Function | [Isoform 1]: Serine protease that shows proteolytic activity against a non-specific substrate beta-casein (PubMed:10873535). Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity; or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism (PubMed:15200957). Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6 (PubMed:36758104, PubMed:36758105). Cleaves THAP5 and promotes its degradation during apoptosis (PubMed:19502560). |
| Cellular Location | Mitochondrion intermembrane space. Mitochondrion membrane; Single-pass membrane protein Note=Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID |
| Tissue Location | [Isoform 1]: Ubiquitously expressed. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq].
References
Zurawa-Janicka, D., et al. Expert Opin. Ther. Targets 14(7):665-679(2010)
Kawamoto, Y., et al. Neuropathol. Appl. Neurobiol. 36(4):331-344(2010)
Vande Walle, L., et al. Cell Res. 20(4):421-433(2010)
Hartkamp, J., et al. Mol. Cell 37(2):159-171(2010)
Kruger, R., et al. Neurobiol. Aging (2009) In press :
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