SYT1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
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Primary Accession | P21579 |
Other Accession | P21707, P46096, Q60HC0, P47191, P48018, NP_001129278.1, NP_005630.1, NP_001129277.1 |
Reactivity | Human |
Predicted | Bovine, Chicken, Monkey, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 47573 Da |
Antigen Region | 349-377 aa |
Gene ID | 6857 |
---|---|
Other Names | Synaptotagmin-1, Synaptotagmin I, SytI, p65, SYT1, SVP65, SYT |
Target/Specificity | This SYT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349-377 amino acids from the C-terminal region of human SYT1. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SYT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SYT1 (HGNC:11509) |
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Synonyms | SVP65, SYT |
Function | Calcium sensor that participates in triggering neurotransmitter release at the synapse (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)- dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed:23999003). |
Cellular Location | Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000250|UniProtKB:P21707}; Single-pass membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:P21707}; Single-pass membrane protein {ECO:0000250|UniProtKB:P21707}. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane {ECO:0000250|UniProtKB:P21707}; Single-pass membrane protein {ECO:0000250|UniProtKB:P21707}. Cytoplasm {ECO:0000250|UniProtKB:P21707} |
Tissue Location | Expressed in melanocytes (PubMed:23999003). |
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Provided below are standard protocols that you may find useful for product applications.
Background
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Musch, M.W., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 298 (2), G203-G211 (2010) :
Kathir, K.M., et al. Biochim. Biophys. Acta 1798(2):297-302(2010)
Pattaro, C., et al. BMC Med. Genet. 11, 41 (2010) :
Hamdan, F.F., et al. Ann. Neurol. 65(6):748-753(2009)
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