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STAT4 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, IF, WB, E |
|---|---|
| Primary Accession | Q14765 |
| Other Accession | P42228, NP_003142.1 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 85941 Da |
| Antigen Region | 666-695 aa |
| Gene ID | 6775 |
|---|---|
| Other Names | Signal transducer and activator of transcription 4, STAT4 |
| Target/Specificity | This STAT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 666-695 amino acids from the C-terminal region of human STAT4. |
| Dilution | IHC-P~~1:10~50 IF~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | STAT4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | STAT4 |
|---|---|
| Function | Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response (PubMed:10961885, PubMed:37256972, PubMed:8943379). Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma (PubMed:12213961, PubMed:35614130). Also participates in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps (By similarity). After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated (PubMed:10415122, PubMed:7638186). Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. Although IL12 appears to be the predominant activating signal, STAT4 can also be phosphorylated and activated in response to IFN-gamma stimulation via JAK1 and TYK2 and in response to different interleukins including IL23, IL2 and IL35 (PubMed:11114383, PubMed:34508746). Transcription activation of IFN-gamma gene is mediated by interaction with JUN that forms a complex that efficiently interacts with the AP-1-related sequence of the IFN-gamma promoter (By similarity). In response to IFN- alpha/beta signaling, acts as a transcriptional repressor and suppresses IL5 and IL13 mRNA expression during response to T-cell receptor (TCR) activation (PubMed:26990433). |
| Cellular Location | Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.
References
Plant, D., et al. Ann. Rheum. Dis. 69(8):1548-1553(2010)
Gestermann, N., et al. Genes Immun. 11(5):432-438(2010)
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Vuong, M.T., et al. PLoS ONE 5 (5), E10559 (2010) :
Glas, J., et al. PLoS ONE 5 (4), E10373 (2010) :
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