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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CTTN Antibody (Center)   

CTTN Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CTTN Antibody (Center) AP13727c
    CTTN Antibody (Center) (Cat. #AP13727c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CTTN antibody detected the CTTN protein (arrow).
    detail
  • WB - CTTN Antibody (Center) AP13727c
    Western blot analysis of CTTN (arrow) using rabbit polyclonal CTTN Antibody (Center) (Cat. #AP13727c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTTN gene.
    detail
  • IHC-P - CTTN Antibody (Center) AP13727c
    CTTN Antibody (Center) (Cat. #AP13727c)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTTN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q14247
Other Accession Q01406, NP_005222.2
Reactivity Human
Predicted Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 61586 Da
Antigen Region 273-302 aa
Additional Information
Gene ID 2017
Other Names Src substrate cortactin, Amplaxin, Oncogene EMS1, CTTN, EMS1
Target/Specificity This CTTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-302 amino acids from the Central region of human CTTN.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTTN Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTTN
Synonyms EMS1
Function Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:21296879). Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (PubMed:20861316). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (PubMed:23144454). Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed:16636290).
Cellular Location Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, dendrite. Cell projection {ECO:0000250|UniProtKB:Q66HL2}. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, podosome {ECO:0000250|UniProtKB:Q01406}. Cell junction {ECO:0000250|UniProtKB:Q66HL2}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q66HL2}. Membrane, clathrin-coated pit {ECO:0000250|UniProtKB:Q66HL2}. Cell projection, dendritic spine. Cytoplasm, cell cortex Endoplasmic reticulum {ECO:0000250|UniProtKB:Q01406}. Note=Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity) Associated with membrane ruffles and lamellipodia. In the presence of CTTNBP2NL, colocalizes with stress fibers (By similarity). In the presence of CTTNBP2, localizes at the cell cortex (By similarity). In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft (By similarity). Colocalizes with DNM2 at the basis of filopodia in hippocampus neuron growth zones (By similarity). {ECO:0000250|UniProtKB:Q60598, ECO:0000250|UniProtKB:Q66HL2}
Research Areas
Citations (0)
citation

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Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

References

Croucher, D.R., et al. Mol. Cell. Biol. 30(21):5057-5070(2010)
Eiseler, T., et al. J. Biol. Chem. 285(24):18672-18683(2010)
Xu, X.Z., et al. Mod. Pathol. 23(2):187-196(2010)
Saitoh, Y., et al. Int. J. Oncol. 35(6):1277-1288(2009)
Hirakawa, H., et al. Int. J. Oncol. 35(6):1271-1276(2009)

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$ 182.50
$ 70.00
Cat# AP13727c
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