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PPP4C Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P60510 |
| Other Accession | Q6IP91, Q5BJ92, P11084, P97470, A6H772, A9JRC7, A8WGP3, NP_002711.1 |
| Reactivity | Human |
| Predicted | Zebrafish, Bovine, Mouse, Rabbit, Rat, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 35080 Da |
| Antigen Region | 160-189 aa |
| Gene ID | 5531 |
|---|---|
| Other Names | Serine/threonine-protein phosphatase 4 catalytic subunit, PP4C, Pp4, Protein phosphatase X, PP-X, PPP4C, PPP4, PPX |
| Target/Specificity | This PPP4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the Central region of human PPP4C. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PPP4C Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PPP4C |
|---|---|
| Synonyms | PPP4, PPX |
| Function | Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2- PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin. |
| Cellular Location | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
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Provided below are standard protocols that you may find useful for product applications.
Background
Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity).
References
Lee, D.H., et al. Nat. Struct. Mol. Biol. 17(3):365-372(2010)
Mourtada-Maarabouni, M., et al. Leuk. Res. 33(11):1539-1551(2009)
Kumar, R.A., et al. PLoS ONE 4 (2), E4582 (2009) :
Chen, G.I., et al. J. Biol. Chem. 283(43):29273-29284(2008)
Nakada, S., et al. EMBO Rep. 9(10):1019-1026(2008)
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