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CBX5 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P45973 |
| Other Accession | Q61686, NP_036249.1, NP_001120794.1, NP_001120793.1 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 22225 Da |
| Antigen Region | 88-117 aa |
| Gene ID | 23468 |
|---|---|
| Other Names | Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha, CBX5, HP1A |
| Target/Specificity | This CBX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human CBX5. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CBX5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CBX5 |
|---|---|
| Synonyms | HP1A |
| Function | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
| Cellular Location | Nucleus. Chromosome. Chromosome, centromere Note=Colocalizes with HNRNPU in the nucleus (PubMed:19617346) Component of centromeric and pericentromeric heterochromatin Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase (PubMed:19617346) Localizes to sites of DNA damage (PubMed:28977666) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
References
Nozawa, R.S., et al. Nat. Cell Biol. 12(7):719-727(2010)
Zeng, W., et al. Epigenetics 5(4):287-292(2010)
Emelyanov, A.V., et al. J. Biol. Chem. 285(20):15027-15037(2010)
Kiyomitsu, T., et al. J. Cell Biol. 188(6):791-807(2010)
Chaturvedi, P., et al. PLoS ONE 5 (5), E10620 (2010) :
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