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NPFFR2 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9Y5X5 |
| Other Accession | NP_004876.2, NP_444264.1, NP_001138228.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 60270 Da |
| Antigen Region | 3-32 aa |
| Gene ID | 10886 |
|---|---|
| Other Names | Neuropeptide FF receptor 2, G-protein coupled receptor 74, G-protein coupled receptor HLWAR77, Neuropeptide G-protein coupled receptor, NPFFR2, GPR74, NPFF2, NPGPR |
| Target/Specificity | This NPFFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-32 amino acids from the N-terminal region of human NPFFR2. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NPFFR2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NPFFR2 (HGNC:4525) |
|---|---|
| Synonyms | GPR74, NPFF2, NPGPR |
| Function | Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. |
| Cellular Location | Cell membrane; Multi-pass membrane protein |
| Tissue Location | Isoform 1 is abundant in placenta. Relatively highly expressed in thymus, testis, and small intestine. Expressed at low levels in several tissues including spleen, prostate, brain, heart, ovary, colon, kidney, lung, liver and pancreas and not expressed in skeletal muscle and leukocytes. Isoform 2 expression is highest in placenta (but at relatively low level compared to isoform 1). Very low level of expression in numerous tissues including adipose tissue and many brain regions. Isoform 3 is expressed in brain and heart and, at lower levels, in kidney, liver, lung and pancreas |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of a subfamily of G-protein-coupled neuropeptide receptors. This protein is activated by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may function in pain modulation and regulation of the opioid system. Alternative splicing results in multiple transcript variants.
References
Talmont, F., et al. Peptides 31(2):215-220(2010)
Goncharuk, V., et al. Peptides 29(9):1544-1553(2008)
Dahlman, I., et al. Am. J. Hum. Genet. 80(6):1115-1124(2007)
Anko, M.L., et al. FEBS Lett. 580(30):6955-6960(2006)
Dowal, L., et al. J. Biol. Chem. 281(33):23999-24014(2006)
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