PTF1A Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q7RTS3 |
Other Accession | NP_835455.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 34970 Da |
Antigen Region | 287-316 aa |
Gene ID | 256297 |
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Other Names | Pancreas transcription factor 1 subunit alpha, Class A basic helix-loop-helix protein 29, bHLHa29, Pancreas-specific transcription factor 1a, bHLH transcription factor p48, p48 DNA-binding subunit of transcription factor PTF1, PTF1-p48, PTF1A, BHLHA29, PTF1P48 |
Target/Specificity | This PTF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-316 amino acids from the C-terminal region of human PTF1A. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PTF1A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PTF1A |
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Synonyms | BHLHA29, PTF1P48 |
Function | Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates. |
Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}. Cytoplasm. Note=In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex (By similarity). |
Tissue Location | Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers |
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Background
This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers.
References
Meredith, D.M., et al. J. Neurosci. 29(36):11139-11148(2009)
Rodolosse, A., et al. Biochem. J. 418(2):463-473(2009)
Tutak, E., et al. Genet. Couns. 20(2):147-152(2009)
Yamada, M., et al. J. Neurosci. 27(41):10924-10934(2007)
Beres, T.M., et al. Mol. Cell. Biol. 26(1):117-130(2006)
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