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PDCD4 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P-Leica, WB, E |
|---|---|
| Primary Accession | Q53EL6 |
| Other Accession | Q9JID1, Q61823, NP_055271.2, NP_663314.1 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 51735 Da |
| Antigen Region | 121-149 aa |
| Gene ID | 27250 |
|---|---|
| Other Names | Programmed cell death protein 4, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Protein 197/15a, PDCD4, H731 |
| Target/Specificity | This PDCD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-149 amino acids from the Central region of human PDCD4. |
| Dilution | WB~~1:2000 IHC-P-Leica~~1:500 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PDCD4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PDCD4 |
|---|---|
| Synonyms | H731 |
| Function | Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity). |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:Q61823}. Cytoplasm {ECO:0000250|UniProtKB:Q61823}. Note=Shuttles between the nucleus and cytoplasm (By similarity). Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133) |
| Tissue Location | Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified.
References
Hayashi, A., et al. Hum. Pathol. 41(11):1507-1515(2010)
Zhang, X., et al. Cancer Sci. 101(10):2163-2170(2010)
Wang, W., et al. Exp. Cell Res. 316(15):2456-2464(2010)
Santhanam, A.N., et al. Oncogene 29(27):3921-3932(2010)
Fassan, M., et al. Oncol. Rep. 24(1):135-139(2010)
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