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IBSP Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P21815 |
| Other Accession | NP_004958.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 35148 Da |
| Antigen Region | 70-99 aa |
| Gene ID | 3381 |
|---|---|
| Other Names | Bone sialoprotein 2, Bone sialoprotein II, BSP II, Cell-binding sialoprotein, Integrin-binding sialoprotein, IBSP, BNSP |
| Target/Specificity | This IBSP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the N-terminal region of human IBSP. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | IBSP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | IBSP |
|---|---|
| Synonyms | BNSP |
| Function | Binds tightly to hydroxyapatite (PubMed:11459848). Appears to form an integral part of the mineralized matrix (PubMed:1818768). Probably important to cell-matrix interaction (PubMed:1818768). Promotes adhesion and migration of various cells via the alpha-V/beta-3 integrin receptor (ITGAV:ITGB3) (PubMed:10640428, PubMed:11459848, PubMed:24103036). |
| Cellular Location | Secreted. |
| Tissue Location | Expressed in bone (at protein level) (PubMed:11459848). Expressed in trophoblast cells of placenta (at protein level) (PubMed:1818768). Expressed in brain (PubMed:36261010) |
Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
References
Giroux, S., et al. Bone 47(5):975-981(2010)
Koller, D.L., et al. J. Clin. Endocrinol. Metab. 95(4):1802-1809(2010)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Gordon, J.A., et al. J. Cell. Biochem. 107(6):1118-1128(2009)
Hwang, Q., et al. BMC Cancer 9, 121 (2009) :
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