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Mouse Ksr1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q61097 |
| Other Accession | NP_038599.1 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 96755 Da |
| Antigen Region | 719-747 aa |
| Gene ID | 16706 |
|---|---|
| Other Names | Kinase suppressor of Ras 1, mKSR1, Protein Hb, Ksr1, Ksr |
| Target/Specificity | This Mouse Ksr1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 719-747 amino acids from the C-terminal region of mouse Ksr1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Ksr1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Ksr1 |
|---|---|
| Synonyms | Ksr |
| Function | Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (PubMed:10409742, PubMed:12932319, PubMed:21102438, PubMed:21441104). Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (By similarity). Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (PubMed:21102438). Its kinase activity is unsure (PubMed:21441104). Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (PubMed:21441104). |
| Cellular Location | Cytoplasm. Membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Cell projection, ruffle membrane. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q8IVT5}. Note=In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. |
| Tissue Location | Expressed in brain, spleen and testis. Isoform 1 is highly expressed spleen and weakly in testis, and isoform 2 is highly expressed in brain and weakly in testis. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
References
Costanzo-Garvey, D.L., et al. Cell Metab. 10(5):366-378(2009)
McKay, M.M., et al. Proc. Natl. Acad. Sci. U.S.A. 106(27):11022-11027(2009)
Razidlo, G.L., et al. J. Biol. Chem. 284(11):6705-6715(2009)
Giurisato, E., et al. Mol. Cell. Biol. 29(6):1554-1564(2009)
Casar, B., et al. Mol. Cell. Biol. 29(5):1338-1353(2009)
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