GTF2H1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | P32780 |
Other Accession | Q9DBA9, NP_001135779.1, NP_005307.1 |
Reactivity | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 62032 Da |
Antigen Region | 84-112 aa |
Gene ID | 2965 |
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Other Names | General transcription factor IIH subunit 1, Basic transcription factor 2 62 kDa subunit, BTF2 p62, General transcription factor IIH polypeptide 1, TFIIH basal transcription factor complex p62 subunit, GTF2H1, BTF2 |
Target/Specificity | This GTF2H1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-112 amino acids from the N-terminal region of human GTF2H1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | GTF2H1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GTF2H1 |
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Synonyms | BTF2 |
Function | Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. |
Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
References
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Wu, W., et al. Lung Cancer 63(2):180-186(2009)
Bethke, L., et al. J. Natl. Cancer Inst. 100(4):270-276(2008)
Di Lello, P., et al. Biochemistry 44(21):7678-7686(2005)
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