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POLR2J Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P52435 |
| Other Accession | Q32P79, NP_006225.1 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 13293 Da |
| Antigen Region | 89-117 aa |
| Gene ID | 5439 |
|---|---|
| Other Names | DNA-directed RNA polymerase II subunit RPB11-a, RNA polymerase II subunit B11-a, RPB11a, DNA-directed RNA polymerase II subunit J-1, RNA polymerase II 133 kDa subunit, POLR2J, POLR2J1 |
| Target/Specificity | This POLR2J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the C-terminal region of human POLR2J. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | POLR2J Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POLR2J |
|---|---|
| Synonyms | POLR2J1 |
| Function | Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. |
| Cellular Location | Nucleus. |
| Tissue Location | Ubiquitously expressed. High expression was found in heart and skeletal muscle. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.
References
Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Shpakovskii, D.G., et al. Bioorg. Khim. 30(6):621-625(2004)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003)
Kaehlcke, K., et al. Mol. Cell 12(1):167-176(2003)
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