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M Mlkl Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - M Mlkl Antibody (C-term) AP14272B
    Immunohistochemical analysis of paraffin-embedded Human tonsil section using M Mlkl antibody(Cat#AP14272b). AP14272b was diluted at 1~400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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  • WB - M Mlkl Antibody (C-term) AP14272B
    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution Lane 1: mouse lung lysates Lane 2: NIH/3T3 whole cell lysates Lane 3: mouse liver lysates Lane 4: mouse testis whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - M Mlkl Antibody (C-term) AP14272B
    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution Lane 1: mouse testis lysates Lane 2: mouse liver lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - M Mlkl Antibody (C-term) AP14272B
    Western blot analysis of lysate from mouse lung tissue lysate, using Mlkl Antibody (C-term)(Cat. #AP14272b). AP14272b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
    detail
  • WB - M Mlkl Antibody (C-term) AP14272B
    All lanes: Anti-M Mlkl Antibody (C-term) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 53KDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • SPECIFICATION
  • CITATIONS: 33
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9D2Y4
Other Accession NP_083281.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 54317 Da
Antigen Region 444-472 aa
Additional Information
Gene ID 74568
Other Names Mixed lineage kinase domain-like protein, Mlkl {ECO:0000312|EMBL:AAH237551, ECO:0000312|MGI:MGI:1921818}
Target/Specificity This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl.
Dilution IHC-P~~1~400
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsM Mlkl Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Mlkl {ECO:0000303|PubMed:23835476, ECO:0000312|MGI:MGI:1921818}
Function Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:23835476, PubMed:27321907, PubMed:24012422, PubMed:24019532, PubMed:32200799, PubMed:32296175). Does not have protein kinase activity (PubMed:24012422). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:23835476, PubMed:27321907, PubMed:24012422, PubMed:24019532). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (PubMed:32200799, PubMed:32296175). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (By similarity).
Cellular Location Cytoplasm. Cell membrane. Nucleus. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (By similarity). Localizes to the nucleus in response to orthomyxoviruses infection (PubMed:32200799). {ECO:0000250|UniProtKB:Q8NB16, ECO:0000269|PubMed:32200799}
Tissue Location Highly expressed in thymus, colon, intestine, liver, spleen and lung. Expressed at much lower level in skeletal muscle, heart and kidney. Not detected in brain
Research Areas
Citations ( 0 )

Background

The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.

References

Bisson, N., et al. Cell Cycle 7(7):909-916(2008)

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$ 365.00
$ 140.00
Cat# AP14272B
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