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APG10L Antibody (C-term S116)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9H0Y0 |
| Other Accession | Q8R1P4, NP_001124500.1 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 25279 Da |
| Antigen Region | 96-121 aa |
| Gene ID | 83734 |
|---|---|
| Other Names | Ubiquitin-like-conjugating enzyme ATG10, 632-, Autophagy-related protein 10, APG10-like, ATG10, APG10L |
| Target/Specificity | This APG10L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-121 amino acids of human APG10L. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | APG10L Antibody (C-term S116) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ATG10 |
|---|---|
| Synonyms | APG10L |
| Function | E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis. |
| Cellular Location | Cytoplasm. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Criollo, A., et al. Cell Death Differ. 14(5):1029-1039(2007)
Shao, Y., et al. Autophagy 3(1):10-16(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Boya, P., et al. Mol. Cell. Biol. 25(3):1025-1040(2005)
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