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SHMT1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P34896 |
| Other Accession | Q5E9P9, NP_004160.3, NP_683718.1, P35623 |
| Reactivity | Human |
| Predicted | Bovine, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 53083 Da |
| Antigen Region | 19-47 aa |
| Gene ID | 6470 |
|---|---|
| Other Names | Serine hydroxymethyltransferase, cytosolic, SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT1 |
| Target/Specificity | This SHMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-47 amino acids from the N-terminal region of human SHMT1. |
| Dilution | IHC-P~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SHMT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SHMT1 |
|---|---|
| Function | Interconversion of serine and glycine (PubMed:24698160, PubMed:8505317). |
| Cellular Location | Cytoplasm. |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.
References
Porter, K.E., et al. Environ. Res. 110(6):580-587(2010)
Summers, C.M., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(8):679-688(2010)
Vijayakrishnan, J., et al. Haematologica 95(8):1405-1414(2010)
Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
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