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Mouse Mapkapk5 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O54992 |
| Other Accession | Q8IW41, XP_001479292.1, NP_034895.1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54152 Da |
| Antigen Region | 39-66 aa |
| Gene ID | 17165 |
|---|---|
| Other Names | MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, Mapkapk5 |
| Target/Specificity | This Mouse Mapkapk5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the N-terminal region of mouse Mapkapk5. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Mapkapk5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Mapkapk5 |
|---|---|
| Function | Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. |
| Cellular Location | Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export |
| Tissue Location | Expressed ubiquitously. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Mapkapk5 mediates stress-induced small heat shock protein 27 phosphorylation (By similarity).
References
Dingar, D., et al. Cell. Signal. 22(7):1063-1075(2010)
Gerits, N., et al. Cell. Mol. Biol. Lett. 14(4):548-574(2009)
Li, Q., et al. J. Biol. Chem. 283(16):11014-11023(2008)
Sun, P., et al. Cell 128(2):295-308(2007)
Seternes, O.M., et al. EMBO J. 23(24):4780-4791(2004)
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$ 365.00
$ 140.00
Cat# AP14447a
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