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Mouse Prpf4b Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q61136 |
| Other Accession | NP_038858.2 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 116976 Da |
| Antigen Region | 180-207 aa |
| Gene ID | 19134 |
|---|---|
| Other Names | Serine/threonine-protein kinase PRP4 homolog, PRP4 pre-mRNA-processing factor 4 homolog, Pre-mRNA protein kinase, Prpf4b, Cbp143, Prp4h, Prp4k, Prp4m, Prpk |
| Target/Specificity | This Mouse Prpf4b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-207 amino acids from the N-terminal region of mouse Prpf4b. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Prpf4b Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Prp4k |
|---|---|
| Synonyms | Cbp143, Prp4h, Prp4m, Prpf4b, Prpk |
| Function | Serine/threonine kinase involved in spliceosomal assembly as well as mitosis and signaling regulation. Connects chromatin mediated regulation of transcription and pre-mRNA splicing. During spliceosomal assembly, interacts with and phosphorylates PRPF6 and PRPF31, components of the U4/U6-U5 tri-small nuclear ribonucleoprotein (snRNP), to facilitate the formation of the spliceosome B complex. Plays a role in regulating transcription and the spindle assembly checkpoint (SAC). Associates with U5 snRNP and NCOR1 deacetylase complexes which may allow a coordination of pre-mRNA splicing with chromatin remodeling events involved in transcriptional regulation. Associates and probably phosphorylates SMARCA4 and NCOR1. Phosphorylates SRSF1. Associates with kinetochores during mitosis and is necessary for recruitment and maintenance of the checkpoint proteins such as MAD1L1 and MAD12L1 at the kinetochores. Phosphorylates and regulates the activity of the transcription factors such as ELK1 and KLF13. Phosphorylates nuclear YAP1 and WWTR1/TAZ which induces nuclear exclusion and regulates Hippo signaling pathway, involved in tissue growth control. |
| Cellular Location | Nucleus. Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q13523} Note=Located throughout the nucleus, excluding the nucleolus but enriched in multiple speckles. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Prpf4b has a role in pre-mRNA splicing. Phosphorylates SF2/ASF.
References
Hoffman, B.G., et al. Genome Biol. 9 (6), R99 (2008) :
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Cobellis, G., et al. Nucleic Acids Res. 33 (4), E44 (2005) :
Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)
Stryke, D., et al. Nucleic Acids Res. 31(1):278-281(2003)
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