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SPRED1 Antibody
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, WB, E |
|---|---|
| Primary Accession | Q7Z699 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 50477 Da |
| Gene ID | 161742 |
|---|---|
| Other Names | Sprouty-related, EVH1 domain-containing protein 1, Spred-1, hSpred1, SPRED1 |
| Target/Specificity | This SPRED1 antibody is generated from rabbits immunized with human SPRED1 recombinant protein. |
| Dilution | FC~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SPRED1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPRED1 |
|---|---|
| Function | Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase (By similarity). Negatively regulates hematopoiesis of bone marrow (By similarity). Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (By similarity). Attenuates actin stress fiber formation via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed:18216281). Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (By similarity). |
| Cellular Location | Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Nucleus Note=Localized in cholesterol-rich membrane raft/caveola fractions |
| Tissue Location | Weakly expressed in embryonic cell line HEK293. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
SPRED1 is a tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.
References
Brems,H., Nat. Genet. 39 (9), 1120-1126 (2007)
Szafranski,K., Genome Biol. 8 (8), R154 (2007)
Yoshida,T., Oncogene 25 (45), 6056-6066 (2006)
King,J.A., Biochem. J. 388 (PT 2), 445-454 (2005)
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