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ASAP1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9ULH1 |
| Other Accession | Q1AAU6, Q9QWY8, O97902, NP_060952.2 |
| Reactivity | Mouse |
| Predicted | Bovine, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 125498 Da |
| Antigen Region | 360-388 aa |
| Gene ID | 50807 |
|---|---|
| Other Names | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130 kDa phosphatidylinositol 4, 5-bisphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, DEF-1, Differentiation-enhancing factor 1, PIP2-dependent ARF1 GAP, ASAP1, DDEF1, KIAA1249 |
| Target/Specificity | This ASAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-388 amino acids from the Central region of human ASAP1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ASAP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ASAP1 |
|---|---|
| Synonyms | DDEF1, KIAA1249, PAG2 {ECO:0000303|PubMe |
| Function | Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Part of the ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which direct preciliary vesicle trafficking to mother centriole and ciliogenesis initiation (PubMed:25673879). |
| Cellular Location | Cytoplasm. Membrane. Golgi apparatus. Golgi apparatus, trans- Golgi network. Note=Predominantly cytoplasmic. Partially membrane-associated. Localized to the Golgi, TGN and rhodopsin transport carriers (RTC) when interacting with RHO in photoreceptors (PubMed:25673879). Localized to RTC when interacting with RAB11A and RAB11FIP3 in photoreceptors (PubMed:25673879) {ECO:0000250, ECO:0000269|PubMed:25673879} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity).
References
Muller, T., et al. Oncogene 29(16):2393-2403(2010)
Nat. Genet. 41(7):824-828(2009)
Jian, X., et al. J. Biol. Chem. 284(3):1652-1663(2009)
Inoue, H., et al. Mol. Biol. Cell 19(10):4224-4237(2008)
Lin, D., et al. Cancer Res. 68(11):4352-4359(2008)
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