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PRPF31 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PRPF31 Antibody (C-term) AP14542b
    PRPF31 Antibody (C-term) (Cat. #AP14542b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PRPF31 antibody detected the PRPF31 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8WWY3
Other Accession Q5U5C5, Q8CCF0, Q7SXM7, NP_056444.3
Reactivity Human
Predicted Zebrafish, Mouse, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 55456 Da
Antigen Region 334-361 aa
Additional Information
Gene ID 26121
Other Names U4/U6 small nuclear ribonucleoprotein Prp31, Pre-mRNA-processing factor 31, Serologically defined breast cancer antigen NY-BR-99, U4/U6 snRNP 61 kDa protein, Protein 61K, hPrp31, PRPF31, PRP31
Target/Specificity This PRPF31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 334-361 amino acids from the C-terminal region of human PRPF31.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRPF31 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRPF31
Synonyms PRP31
Function Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11867543, PubMed:28781166). Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed:11867543).
Cellular Location Nucleus. Nucleus speckle. Nucleus, Cajal body. Note=Predominantly found in speckles and in Cajal bodies.
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)
citation

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Background

This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.

References

Schneider, M., et al. Nat. Struct. Mol. Biol. 17(2):216-221(2010)
Bujakowska, K., et al. Invest. Ophthalmol. Vis. Sci. 50(12):5927-5933(2009)
Potkin, S.G., et al. PLoS ONE 4 (8), E6501 (2009) :
Liu, S., et al. Science 316(5821):115-120(2007)
Sullivan, L.S., et al. Invest. Ophthalmol. Vis. Sci. 47(10):4579-4588(2006)

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$ 182.50
$ 70.00
Cat# AP14542b
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