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USP30 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P-Leica, WB, E |
|---|---|
| Primary Accession | Q70CQ3 |
| Other Accession | D3ZPG5, Q3UN04, NP_116052.2 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 58503 Da |
| Antigen Region | 156-185 aa |
| Gene ID | 84749 |
|---|---|
| Other Names | Ubiquitin carboxyl-terminal hydrolase 30, Deubiquitinating enzyme 30, Ubiquitin thioesterase 30, Ubiquitin-specific-processing protease 30, Ub-specific protease 30, USP30 |
| Target/Specificity | This USP30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the N-terminal region of human USP30. |
| Dilution | IHC-P-Leica~~1:500 WB~~1:2000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | USP30 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | USP30 (HGNC:20065) |
|---|---|
| Function | Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:18287522, PubMed:24896179, PubMed:25527291, PubMed:25621951). Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling (PubMed:25621951). Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (PubMed:25527291). Acts as a negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity). |
| Cellular Location | Mitochondrion outer membrane |
| Tissue Location | Expressed in skeletal muscle, pancreas, liver and kidney. |
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Provided below are standard protocols that you may find useful for product applications.
Background
USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]).
References
Nakamura, N., et al. Mol. Biol. Cell 19(5):1903-1911(2008)
Quesada, V., et al. Biochem. Biophys. Res. Commun. 314(1):54-62(2004)
Puente, X.S., et al. Nat. Rev. Genet. 4(7):544-558(2003)
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