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SCF (KITLG) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - SCF (KITLG) Antibody (C-term) AP1484b
    Immunofluorescence analysis of anti-KITLG Antibody (C-term) (Cat.#AP1484b) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
    detail
  • WB - SCF (KITLG) Antibody (C-term) AP1484b
    Western blot analysis of KITLG Antibody (C-term) (Cat.#AP1484b) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab.
    detail
  • WB - SCF (KITLG) Antibody (C-term) AP1484b
    Western blot analysis of KITLG(arrow) using rabbit polyclonal KITLG Antibody (C-term) (Cat.#AP1484b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies).
    detail
  • FC - SCF (KITLG) Antibody (C-term) AP1484b
    Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IF, WB, E
Primary Accession P21583
Other Accession Q29030, Q28132, Q95MD2
Reactivity Human
Predicted Bovine, Horse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 30899 Da
Antigen Region 244-273 aa
Additional Information
Gene ID 4254
Other Names Kit ligand, Mast cell growth factor, MGF, Stem cell factor, SCF, c-Kit ligand, Soluble KIT ligand, sKITLG, KITLG, MGF, SCF
Target/Specificity This SCF (KITLG) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-273 amino acids from the C-terminal region of human SCF (KITLG).
Dilution IF~~1:10~50
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSCF (KITLG) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KITLG (HGNC:6343)
Synonyms MGF, SCF
Function Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Cellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein [Soluble KIT ligand]: Secreted.
Research Areas
Citations (0)
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Background

KITLG is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis.

References

Young,S.M., Cell. Signal. 19 (12), 2572-2581 (2007)
Pick,M., Stem Cells 25 (9), 2206-2214 (2007)
Yasuda,A., Dig. Dis. Sci. 52 (9), 2292-2300 (2007)

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$ 365.00
$ 140.00
Cat# AP1484b
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