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SECISBP2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96T21 |
| Other Accession | Q9QX72, NP_076982.3, Q3U1C4 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 95462 Da |
| Antigen Region | 501-530 aa |
| Gene ID | 79048 |
|---|---|
| Other Names | Selenocysteine insertion sequence-binding protein 2, SECIS-binding protein 2, SECISBP2, SBP2 |
| Target/Specificity | This SECISBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 501-530 amino acids from the Central region of human SECISBP2. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SECISBP2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SECISBP2 {ECO:0000303|PubMed:19004874, ECO:0000312|HGNC:HGNC:30972} |
|---|---|
| Function | mRNA-binding protein that binds to the SECIS (selenocysteine insertion sequence) element present in the 3'-UTR of mRNAs encoding selenoproteins and facilitates the incorporation of the rare amino acid selenocysteine (PubMed:35709277). Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling (PubMed:35709277). (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation (PubMed:35709277). (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl- tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur (PubMed:35709277). |
| Cellular Location | [Isoform 1]: Nucleus. |
| Tissue Location | Expressed at high levels in testis. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism.
References
Meplan, C., et al. Carcinogenesis 31(6):1074-1079(2010)
Papp, L.V., et al. Antioxid. Redox Signal. 12(7):797-808(2010)
Di Cosmo, C., et al. J. Clin. Endocrinol. Metab. 94(10):4003-4009(2009)
Olieric, V., et al. Biochimie 91(8):1003-1009(2009)
Schomburg, L., et al. Thyroid 19(3):277-281(2009)
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