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FXYD5 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q96DB9 |
| Other Accession | NP_001158077.1, NP_659003.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 19472 Da |
| Antigen Region | 71-100 aa |
| Gene ID | 53827 |
|---|---|
| Other Names | FXYD domain-containing ion transport regulator 5, Dysadherin, FXYD5, DYSAD, IWU1 |
| Target/Specificity | This FXYD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-100 amino acids from the Central region of human FXYD5. |
| Dilution | IHC-P~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | FXYD5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | FXYD5 |
|---|---|
| Synonyms | DYSAD, IWU1 |
| Function | Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane (By similarity). May increase NKA activity by increasing the apparent affinity for Na(+) (PubMed:18263667). Involved in down-regulation of E-cadherin which results in reduced cell adhesion. Promotes metastasis (PubMed:11756660). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P97808} Basolateral cell membrane {ECO:0000250|UniProtKB:P97808}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P97808}. Note=In kidneys localizes to the basolateral membrane of the connecting tubule {ECO:0000250|UniProtKB:P97808} |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product, FXYD5, is a glycoprotein that functions in the up-regulation of chemokine production, and it is involved in the reduction of cell adhesion via its ability to down-regulate E-cadherin. It also promotes metastasis, and has been linked to a variety of cancers. Alternative splicing results in multiple transcript variants. [RefSeq curation by Kathleen J. Sweadner, Ph.D., sweadner@helix.mgh.harvard.edu.].
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Ono, K., et al. Anticancer Res. 30(9):3273-3278(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Liang, J.F., et al. Pathol. Res. Pract. 205(7):445-450(2009)
Batistatou, A., et al. Endocr. Pathol. 19(3):197-202(2008)
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