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Actin (ACTB/ACTC) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - Actin (ACTB/ACTC) Antibody (N-term) AP1491a
    Confocal immunofluorescent analysis of Actin (ACTB/ACTC) Antibody (N-term)(Cat#AP1491a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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  • WB - Actin (ACTB/ACTC) Antibody (N-term) AP1491a
    Western blot analysis of ACTB/ACTC Antibody (N-term) in mouse testis tissue lysates (35ug/lane). Target protein (arrow) was detected using the purified Pab.
    detail
  • IHC-P - Actin (ACTB/ACTC) Antibody (N-term) AP1491a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACTB/ACTC antibody (N-term) (Cat.#AP1491a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
  • CITATIONS: 4
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P68032
Other Accession P60010, P68136, P68135, P68137, P68134, P68133, P68139, P68138, P63269, P63268, P63267, P63270, Q5E9B5, A2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P04751, P68035, P68033, P68034, Q3ZC07, O93400, P60711, P29751, Q6QAQ1, P60710, Q4R561, P60709, P48975
Reactivity Human, Mouse
Predicted C.Elegans, Drosophila, Xenopus, Chicken, Bovine, Rabbit, Rat, Zebrafish, Hamster, Horse, Monkey, Pig, Sheep, Yeast
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 42019 Da
Antigen Region 38-67 aa
Additional Information
Gene ID 70
Other Names Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTC1, ACTC
Target/Specificity This Actin (ACTB/ACTC) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-67 amino acids from the N-terminal region of human Actin (ACTB/ACTC).
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsActin (ACTB/ACTC) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTC1
Synonyms ACTC
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations ( 0 )

Background

Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

References

Villebeck,L., Biochemistry 46 (44), 12639-12647 (2007) Avizienyte,E., Exp. Cell Res. 313 (15), 3175-3188 (2007) Bouldin,A.A., Muscle Nerve 35 (2), 254-258 (2007)

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$ 365.00
$ 140.00
Cat# AP1491a
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