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USP12 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O75317 |
| Other Accession | Q9D9M2, A5D9H7, Q5M981, Q52KZ6, NP_872294.2 |
| Reactivity | Mouse |
| Predicted | Xenopus, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 42858 Da |
| Antigen Region | 222-251 aa |
| Gene ID | 219333 |
|---|---|
| Other Names | Ubiquitin carboxyl-terminal hydrolase 12, Deubiquitinating enzyme 12, Ubiquitin thioesterase 12, Ubiquitin-hydrolyzing enzyme 1, Ubiquitin-specific-processing protease 12, USP12, UBH1, USP12L1 |
| Target/Specificity | This USP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-251 amino acids from the Central region of human USP12. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | USP12 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | USP12 |
|---|---|
| Synonyms | UBH1, USP12L1 |
| Function | Deubiquitinating enzyme that plays various roles in the regulation of the immune response and inflammation (PubMed:19075014, PubMed:27373336). In complex with WDR48, acts as a potential tumor suppressor by positively regulating PHLPP1 stability (PubMed:24145035). During TCR engagement and activation, translocates into the cytoplasm and deubiquitinates its substrates LAT and TRAT1 and prevents their lysosome-dependent degradation to stabilize the TCR signaling complex at the plasma membrane (PubMed:26811477). Plays an essential role in the selective LPS-induced macrophage response through the activation of NF-kappa-B pathway (PubMed:28063927). In addition, promotes that antiviral immune response through targeting DNA sensor IFI16 to inhibit its proteasome-dependent degradation (PubMed:37410794). Participates in the interferon signaling pathway and antiviral response independently of its deubiquitinase activity by maintaining nuclear phosphorylated STAT1 levels via inhibition of its CREBBP-mediated acetylation and subsequent dephosphorylation (PubMed:31899788). Plays an intrinsic role in promoting the differentiation, activation and proliferation of CD4(+) T-cell by activating the NF-kappa-B signaling pathway through deubiquitinating and stabilizing B-cell lymphoma/leukemia 10/BCL10 (By similarity). In myeloid-derived suppressor cells promotes the activation of the NF-kappa-B via deubiquitination and stabilization of RELA (By similarity). Regulates the 'Lys-63'-linked polyubiquitin chains of BAX and thereby modulates the mitochondrial apoptotic process (PubMed:36361894). |
| Cellular Location | Nucleus. Cytoplasm. Cell membrane. Note=Translocates from the nucleus to the cytosol on TCR stimulation, while it translocates into the nucleus in IFN signaling. USP12/WDR20/WDR48 complex is localized mainly to the plasma membrane (PubMed:30466959). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
USP12is a deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.
References
Kee, Y., et al. J. Biol. Chem. 285(15):11252-11257(2010)
Asano, K., et al. Nat. Genet. 41(12):1325-1329(2009)
Cohn, M.A., et al. J. Biol. Chem. 284(8):5343-5351(2009)
Dunham, A., et al. Nature 428(6982):522-528(2004)
Puente, X.S., et al. Nat. Rev. Genet. 4(7):544-558(2003)
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