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CLCNKB Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P51801 |
| Other Accession | Q9WUB7, NP_000076.2 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 75446 Da |
| Antigen Region | 11-40 aa |
| Gene ID | 1188 |
|---|---|
| Other Names | Chloride channel protein ClC-Kb, Chloride channel Kb, ClC-K2, CLCNKB |
| Target/Specificity | This CLCNKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the N-terminal region of human CLCNKB. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CLCNKB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CLCNKB {ECO:0000303|PubMed:18310267, ECO:0000312|HGNC:HGNC:2027} |
|---|---|
| Function | Anion-selective channel permeable to small monovalent anions with ion selectivity for chloride > bromide > nitrate > iodide (PubMed:11734858, PubMed:12111250). Forms a homodimeric channel where each subunit has its own ion conduction pathway. May conduct double- barreled currents controlled by two types of gates, two fast gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously (PubMed:11734858, PubMed:12111250, PubMed:16849430, PubMed:18776122, PubMed:19646679). Assembles with the regulatory subunit BSND/Barttin for sorting at the basolateral plasma membrane domain and functional switch to the ion conducting state. CLCNKB:BSND channels display mostly a linear current- voltage relationship controlled by common gate (PubMed:11734858, PubMed:12111250, PubMed:16849430, PubMed:18776122, PubMed:19646679). Mediates chloride conductance along nephron segments, namely the thick ascending limb of Henle's loop, convoluted tubule and the collecting duct, contributing to the maintenance of systemic acid-base and electrolyte homeostasis (By similarity). Conducts chloride currents in the stria vascularis of the inner ear to establish the endocochlear potential necessary for normal hearing (PubMed:15044642, PubMed:18310267, PubMed:19646679). |
| Cellular Location | Basolateral cell membrane; Multi-pass membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the family of voltage-gated chloride channels. Chloride channels have several functions, including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. This gene is expressed predominantly in the kidney and may be important for renal salt reabsorption. Mutations in this gene are associated with autosomal recessive Bartter syndrome type 3 (BS3). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Yu, Y., et al. Clin. Genet. 77(2):155-162(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Brochard, K., et al. Nephrol. Dial. Transplant. 24(5):1455-1464(2009)
Sile, S., et al. J. Hypertens. 27(2):298-304(2009)
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