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DKK2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DKK2 Antibody (C-term) AP1522b
    DKK2 Antibody (C239) (Cat. #AP1522b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the DKK2 antibody detected the DKK2 protein (arrow).
    detail
  • IHC-P - DKK2 Antibody (C-term) AP1522b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9UBU2
Other Accession Q9QYZ8
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 28447 Da
Antigen Region 225-251 aa
Additional Information
Gene ID 27123
Other Names Dickkopf-related protein 2, Dickkopf-2, Dkk-2, hDkk-2, DKK2
Target/Specificity This DKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-251 amino acids from the C-terminal region of human DKK2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDKK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DKK2
Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
Cellular Location Secreted.
Tissue Location Expressed in heart, brain, skeletal muscle and lung
Research Areas
Citations (0)
citation

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Background

The 259-amino acid DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.

References

Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
Brott, B.K., et al., Mol. Cell. Biol. 22(17):6100-6110 (2002).
Krupnik, V.E., et al., Gene 238(2):301-313 (1999).

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$ 182.50
$ 70.00
Cat# AP1522b
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