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MFN1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q8IWA4 |
| Other Accession | NP_284941.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 84160 Da |
| Antigen Region | 353-381 aa |
| Gene ID | 55669 |
|---|---|
| Other Names | Mitofusin-1, 365-, Fzo homolog, Transmembrane GTPase MFN1, MFN1 |
| Target/Specificity | This MFN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-381 amino acids from the Central region of human MFN1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MFN1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MFN1 |
|---|---|
| Function | Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12475957, PubMed:12759376, PubMed:27920125, PubMed:28114303). Membrane clustering requires GTPase activity (PubMed:27920125). It may involve a major rearrangement of the coiled coil domains (PubMed:27920125, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12475957, PubMed:12759376). Overexpression induces the formation of mitochondrial networks (in vitro) (PubMed:12759376). Has low GTPase activity (PubMed:27920125, PubMed:28114303). |
| Cellular Location | Mitochondrion outer membrane; Multi-pass membrane protein |
| Tissue Location | Detected in kidney and heart (at protein level) (PubMed:12759376). Ubiquitous (PubMed:11950885, PubMed:12759376) Expressed at slightly higher level in kidney and heart (PubMed:12759376). Isoform 2 may be overexpressed in some tumors, such as lung cancers (PubMed:11751411). |
Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting.
References
Perumalsamy, L.R., et al. Proc. Natl. Acad. Sci. U.S.A. 107(15):6882-6887(2010)
Park, Y.Y., et al. J. Cell. Sci. 123 (PT 4), 619-626 (2010) :
Onoguchi, K., et al. PLoS Pathog. 6 (7), E1001012 (2010) :
Waxman, A.B., et al. Am. J. Respir. Cell Mol. Biol. 41(4):385-396(2009)
Wolf, C., et al. BMC Med. Genet. 10, 91 (2009) :
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