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SMO Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SMO Antibody (Center) AP16325c
    SMO Antibody (Center) (Cat. #AP16325c) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SMO antibody detected the SMO protein (arrow).
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Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99835
Other Accession NP_005622.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 86397 Da
Antigen Region 539-567 aa
Additional Information
Gene ID 6608
Other Names Smoothened homolog, SMO, Protein Gx, SMO, SMOH
Target/Specificity This SMO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 539-567 amino acids from the Central region of human SMO.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMO Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMO
Synonyms SMOH
Function G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed:19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P56726}; Multi-pass membrane protein. Cell projection, cilium. Note=Cilium localization is promoted by SHH and is required for activity. {ECO:0000250|UniProtKB:P56726}
Research Areas
Citations ( 0 )


The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.


Zhang, L., et al. Oral Dis 16(8):818-822(2010)
Desch, P., et al. Oncogene 29(35):4885-4895(2010)
Walter, K., et al. Clin. Cancer Res. 16(6):1781-1789(2010)
Hirotsu, M., et al. Mol. Cancer 9, 5 (2010) :
Rittie, L., et al. Aging Cell 8(6):738-751(2009)

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$ 182.50
$ 70.00
Cat# AP16325c
Availability: In Stock
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