DDX53 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND

Application
| WB, E |
|---|---|
| Primary Accession | Q86TM3 |
| Other Accession | NP_874358.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 71154 Da |
| Antigen Region | 590-619 aa |
| Gene ID | 168400 |
|---|---|
| Other Names | Probable ATP-dependent RNA helicase DDX53, Cancer-associated gene protein, Cancer/testis antigen 26, CT26, DEAD box protein 53, DEAD box protein CAGE, DDX53, CAGE |
| Target/Specificity | This DDX53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-619 amino acids from the C-terminal region of human DDX53. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DDX53 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DDX53 |
|---|---|
| Synonyms | CAGE |
| Cellular Location | Nucleus. |
| Tissue Location | Expressed in testis. Wide expression in various cancer tissues and cancer cell lines. |

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Provided below are standard protocols that you may find useful for product applications.
Background
DDX53 belongs to the DEAD box helicase family. It is expressed in testis and shows wide expression in various cancer tissues and cancer cell lines.
References
Kim, Y., et al. J. Biol. Chem. 285(34):25957-25968(2010)
Pinto, D., et al. Nature 466(7304):368-372(2010)
Kim, Y., et al. J. Microbiol. Biotechnol. 18(3):600-610(2008)
Lee, T.S., et al. Ann. N. Y. Acad. Sci. 1091, 218-224 (2006) :
Shim, H., et al. Biotechnol. Lett. 28(24):2057-2063(2006)
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