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ZNF81 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ZNF81 Antibody (N-term) AP16535a
    Fluorescent confocal image of U251 cell stained with ZNF81 Antibody (N-term)(Cat#AP16535a).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF81 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ZNF81 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly.
    detail
  • WB - ZNF81 Antibody (N-term) AP16535a
    ZNF81 Antibody (N-term) (Cat. #AP16535a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ZNF81 antibody detected the ZNF81 protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession P51508
Other Accession NP_009068.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 75960 Da
Antigen Region 169-197 aa
Additional Information
Gene ID 347344
Other Names Zinc finger protein 81, HFZ20, ZNF81
Target/Specificity This ZNF81 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-197 amino acids from the N-terminal region of human ZNF81.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZNF81 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZNF81
Function May be involved in transcriptional regulation.
Cellular Location Nucleus.
Citations (0)
citation

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Background

This gene encodes a protein that likely functions as a transcription factor. The protein contains an N-terminal KRAB domain and several C2H2-type zinc finger motifs. Mutations in this gene cause mental retardation X-linked type 45 (MRX45). [provided by RefSeq].

References

Kleefstra, T., et al. J. Med. Genet. 41(5):394-399(2004)
Hamel, B.C., et al. Am. J. Med. Genet. 85(3):290-304(1999)
Knight, J.C., et al. Genomics 21(1):180-187(1994)
Marino, M., et al. Mamm. Genome 4(5):252-257(1993)

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$ 182.50
$ 70.00
Cat# AP16535a
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